Pakistan has a high disease burden of gastro-enteric infections and it is a serious disease caused by Rotavirus. Diarrheal infections are considered as a significant cause of infant and childhood morbidity and mortality in both developing as well as developed countries, which increases the death risks among children less than 5 years of age. Globally, it is also reported that the children who are less than 5 years are hospitalized every year due to rotavirus infection. This infection attacks the bowel, which causes fever, vomiting, dehydration and water stool (diarrhea). The infection is treatable and can be cured within a week. The cause of diarrhea is poor environment sanitation, inadequate water supply, poverty and inadequate education. This may be transmitted by fecal, oral route and also spread through contaminated food, drinking water and from a person who is infected as a result of unhygienic practices.