Infection control has a strong commitment and with clear responsibilities for effective implementation of standard infection control procedure, including handwashing/hand hygiene, disinfection/sterilization, personal protective equipment, and waste management. The aim of the study is to determine the level of implementation of infection control among nursing staff in the special area and different wards of Tobruk Medical Center in order to generate information regarding infectious disease that can be prevented from spreading through contamination from nursing staff to patient, patient to family, health care provider to family, and The need to understand infection prevention practice is important for prevention and control of nosocomial infection. This study revealed that male (3.31) nurses implement infection control at a higher level than female (3.05) nurses do. The graduates of Bachelor of Science in nursing (3.22) implement infection control at a higher degree than the Nursing Diploma graduates (3.13), it also revealed that hand hygiene/ handwashing (1.75) and waste disposal (2.61) had relatively lower mean score in Medical ward, therefore there is a need to enhance the infection control activities in these two areas. However, there are no significant differences on the level of implementation of infection control program when Nurses are grouped according to their profile. To sustain good practices, adequate pre-service and in-service training should be in place to equip and update health care workers specially nurses about infection prevention precautions. The need for continuous supervision should be implemented to strengthen adherence for infection prevention practices among Health workers along with sustainable and reliable supply