Design and Manufacture of Poly (L-Lactic) Nano Scaffold and Investigating the Effect of Willemite (Zn2SIO4) Nanoparticles in the Process of Equine Adipose-Derived Mesenchymal Stem Cells Differentiation into Bone
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Abstract
Introduction: One of the most common problems among race-horses is the breaking of bones in the foot, which does not produce desirable responses to usual surgical and medicinal treatments. Extraction and purification of stem cells, especially mesenchymal cells, cause a reduction in the costs of treating race-horses, and protecting them in
race-tracks.
Methods: Alkaline phosphatase measurement test, performed to prove the presence of bone progenitor cells, and the MTT test,
Result: The results of analyzing bone indexes showed that nanocomposite Poly-L-lactic-acid-willemite is a suitable ground for cultivating and differentiation of stem cells and bone progenitor cells, and the method used is an efficient method for generating suitable scaffold for bone tissue engineering.