The adhesion of bacterial cells on any floor is the first step of biofilm formation. Biomedical subject is greater challenge approximately biofilm formation as scientific gadgets get frequently contaminated with microorganism and main to the formation of mature biofilm which could positioned patient lives at threat. Researchers have stated that the surface topography with micro patterns impacts the initial adhesion of bacterial cells. However, the effect of the range of corners at the conduct of bacterial cells is yet to be investigated. In this work, the unique kinds of microscale geometrical functions (Triangle, Square, Star pattern with 4 and 5 corners) are fabricated on polydimethylsiloxane (PDMS) the use of smooth lithography technique. Through static droplet touch perspective analysis, its miles confirmed that the fabricated micro patterns modify the wettability of simple PDMS surface. The effect of variety of corners at the migration and preliminary attachment of Escherichia coli (E. coli) and Staphylococcus aureus (S. Aureus) are investigated the use of fluorescence microscopy. The adhesion of bacterium depends at the range of corners present within the geometrical feature. Moreover, the bacterial adhesion is correlated with the ratio of pillar pass-sectional area to its perimeter. The experimental investigation may be useful in designing the powerful geometry which may be used in making anti-biofouling dependent polymeric devices for biomedical applications.