A novel Co(II)-containing metal-organic framework [Co3 (OH)2 (H2 TCPP)2 ](DMF)3 (1) based on a linear trinuclear Co(II) cluster-based unit has been successfully prepared by using a nanosized tetracarboxylate ligand 2,3,5,6-tetrakis(4-carboxyphenyl)pyrazine (H4 TCPP) as the organic building block under the solvothermal reaction. Gas adsorption studies reveal that the mixtures of C2 H2 /CH4 and C2 H2 /CO2 could be selectively separated by the obtained resulting solvent free 1 (denoted as 1a hereafter). To deep evaluate the protective function of complex for AGN disease in vitro, the following experiments were conducted. Firstly, real time RT-PCR was carried out to determine the relative expression of Nf-κβ and tNf-α in the glomerulus cells after bacterial infection along with complex 1 treatment. Then, the ELISA was conducted to determine the IL-1β and IL-6 released by glomerular cells after complex 1 treatment. Finally, the results obtained through molecular docking exhibited possible regulation mechanism on the binding affinity between compound 1 and target protein.